Hoechst 33258 analog 3_23554-98-5_DataSheet_MedChemExpress

Product Data SheetProduct Name:CAS No.: Cat.No.:Cat. No.:MWt:Formula:Purity :Hoechst 33258 analog 323554-98-5HY-15625HY15625422.52C26H26N6>98%Solubility:25°C:25C: DMSO or water Protect fromDMSOorwaterProtectfromlightMechanisms: Pathways:Others; Target:DNA Stain Biological Activity:Description: DitiIC50 Value: N/A Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so thatthe dye Hoechst 33342 is the 33342nd compound made by the company. There are three relatedHoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 andHoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra.Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent lightaroundanemissionmaximumat461nmUnbounddyehasitsmaximumfluorescenceemissioninaround anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission inthe 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethylformamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueoussoluti...References:[1]. Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole andbisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949(2): 158-68. [2]. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acidsynthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : officialjournal of the Histochemistry Society 23 (7): 493-505. [3]. a b c \"Hoechst Stains\". Invitrogren (Molecular Probes). Caution: Not fully tested. For research purposes onlyMedchemexpress LLCwww.medchemexpress.com18Wilkinson Way, Princeton, NJ 08540,USAEmail: info@medchemexpress.com Web: www.medchemexpress.com